It is recommended that cells are initially grown and tested in IROA unlabeled mammalian media, (IROA Cat. No. IROA-300-UL, $200.00), prior to labeling with the IROA 300-250 Mammalian Profiling Kit, to ensure that the IROA media supports the growth and proliferation of your cells.
 
At least 5 or more cell doubling cycles are required to achieve full label incorporation.