The IROA 300 Mammalian profiling kit is a simple and direct metabolic labeling protocol for the in-vivo incorporation of specific and unique signatures or tags into biochemical metabolites for relative quantitation by mass spectrometry (MS). 

 

The IROA protocols can be applied to many life science applications (toxicology, diagnostics, drug development, bioprocess, etc.) as the technique is amenable to most cell populations, including bacterial, yeast and mammalian cell lines. Cell types or organisms labeled with IROA media include: CHO, C. elegans, Dendritic cells, E. coli (common strains and genetically modified), Fibroblasts, HaCat, HC-04, HepG2, HL60, Jurkat, Lactobacillus, Leukemia-like cell lines, Liver cancer cell lines, Macrophages, Mel-3, Myxococcus xanthus, OVAR-8, Plasmodium, and Yeast (common strains and genetically modified).

 

The IROA Quantitation Series 300 kits are designed to grow and label mammalian cell types that require complex nutrient energy sources (labeling is performed with multiple carbon sources) and may not be appropriate for all mammalian cell types, fungal or bacterial populations.