![]() | IROA 300: Mammalian Metabolic Profiling | |
The IROA 300 Mammalian Metabolic Profiling Kit is a simple and direct metabolic labeling protocol for the in-vivo incorporation of specific and unique signatures or tags into biochemical metabolites for relative quantitation by mass spectrometry (MS). The IROA protocols can be applied to many life science applications (toxicology, diagnostics, drug development, bioprocess, etc.) as the technique is amenable to most cell populations, including bacterial, yeast and mammalian cell lines. Cell types or organisms labeled with IROA media include: CHO, C. elegans, Dendritic cells, E. coli (common strains and genetically modified), Fibroblasts, HaCat, HC-04, HepG2, HL60, Jurkat, Lactobacillus, Leukemia-like cell lines, Liver cancer cell lines, Macrophages, Mel-3, Myxococcus xanthus, OVAR-8, Plasmodium, and Yeast (common strains and genetically modified). The IROA Series 300 kits are specific for mammalian cells but may not be appropriate for all cell types. IROA Mammalian Metabolic Profiling Kit Cat No. IROA 300-250 Kit Price: $3594.00 Materials: EBSS/RPMI 1640 vitamins PLUS U-13C, 95% and 5% labeled carbon energy sources; ClusterFinder software Additional Materials Required
For a quote please contact us.To ensure that the IROA media supports the growth and proliferation of your cells, it is recommended that cells are initially grown and tested in IROA unlabeled mammalian media, (IROA Cat. No. IROA-300-UL, $200.00), prior to labeling with the Mammalian profiling kit. At least 5 or more cell doubling cycles are required to achieve full label incorporation. For a quote please contact us. |