MS Standards

Mass Spectrometry Metabolite Library of Standards (MSMLS) supplied with software tool Discovery.

600 high quality metabolite standards, convenient 96 well format, 5 µg per compound

Discovery software tool builds authenic standard libraries by supporting the extraction, manipulation, and storage of the data generated when using MSMLS.

Libraries can be used for sample analysis.


New! Large Scale Metabolite Library of Standards (LSMLS)

504 high quality metabolite standards in convenient 96 well format, 1 mg per compound, plus Discovery software tool
 

 

Discover

How many real metabolites are in your data set?

Have an internal standard for every metabolite you measure - here's how...

Cost effective identification and quantitation of 100’s of analytes simultaneously

  • Unique fully labeled IROA (U- 95% 13C) yeast extract = Internal Standard (IS)
  • IS paired with (U- 5% 13C) yeast extract = Matrix
  • Artifactual, non-paired peaks eliminated, removing false data
  • Software automatically finds, quantitates and IDs corresponding IS compounds in experimental samples
  • Matrix analyzed every 10 samples to create Rentention Index specific to daily chromatographic run for QA/QC 
 

 

New IROA Publication

CLC-HRMS Based Non-Targeted Metabolomic profiling of Wheat (Triticum aestivum L.) under Post-Anthesis Drought Stress

Mohammad Atikur Rahman, Masum Akond, Md Ali Babar, Chris Beecher, John Erickson, Kayla Thomason, Felice A de Jong, Richard Esten Mason

Abstract

Drought stress at the reproductive stage causes severe damage to productivity of wheat. However, little is known about the metabolites associated with drought tolerance. The objectives of this study were to elucidate changes in metabolite levels in wheat under drought, and to identify potential metabolites associated with drought stress through untargeted metabolomic profiling using a liquid chromatography-high resolution mass spectrometry (LC-HRMS)-based technique called Isotopic Ratio Outlier Analysis. Metabolomic analysis was performed on flag leaves of drought-stressed and control (well-watered) plants after 18 days of post-anthesis drought stress at three-hour intervals over a 24-hour period. Out of 723 peaks detected in leaves, 221 were identified as known metabolites. Sixty known metabolites were identified as important metabolites by 3 different methods, PLS-DA, RF and SAM. The most pronounced accumulation due to drought stress was demonstrated by tryptophan, proline, pipecolate and linamarin, whereas the most pronounced decrease was demonstrated by serine, trehalose, N-acetyl-glutamic acid, DIBOA-glucoside etc. Three different patterns of metabolite accumulation were observed over 24-hour period. The increased accumulated metabolites remained higher during all 8 time points in drought stressed leaves. On the contrary, metabolites that showed decreased level remained significantly lower during all or the most time points. However, the levels of some decreased metabolites were lower during the day, but higher during night in drought stressed leaves.
 
Pub. Date: November 15, 2017